Anti-MBD1 Monoclonal Antibody

Product Name

Anti-MBD1 Monoclonal Antibody

View all MBD1 Antibodies

SKU/Catalog Number

M02336

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MBD1 Monoclonal Antibody catalog # M02336. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MBD1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02336)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACAI-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MBD1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02336 is reactive to MBD1 in Human

Applications

M02336 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

67, 80 kDa

Calculated molecular weight

66.607kDa

Background of MBD1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MBD1 using anti-MBD1 antibody (M02336).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified monoclonal antibody (Catalog # M02336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD1 at approximately 67, 80 kDa. The expected band size for MBD1 is at 67 kDa.

Gene/Protein Information For MBD1 (Source: Uniprot.org, NCBI)

Alternative Names

CXXC-type zinc finger protein 3; methyl-CpG binding domain protein 1 isoform PCM1; methyl-CpG binding domain protein 1; methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM1CXXC3RFT; Protein containing methyl-CpG-binding domain 1; the regulator of fibroblast growth factor 2 (FGF-2) transcription MBD1 CXXC3, PCM1, RFT methyl-CpG binding domain protein 1 methyl-CpG-binding domain protein 1|CXXC-type zinc finger protein 3|protein containing methyl-CpG-binding domain 1|the regulator of fibroblast growth factor 2 (FGF-2) transcription

For more info on MBD1, check out the MBD1 Infographic

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In this infographic, you will see the following information for MBD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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